› 7s3d

Electron Microscopy
2.91Å resolution

Structure of photosystem I with bound ferredoxin from Synechococcus sp. PCC 7335 acclimated to far-red light

Released:
DOI: 10.2210/pdb7s3d/pdb
PDB entry 7s3d coloured by chain, front view.
PDB entry 7s3d coloured by chain, side view.
PDB entry 7s3d coloured by chain, top view.
Source organism: Synechococcus sp. PCC 7335
Primary publication:
Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation.
Gisriel CJ, Flesher DA, Shen G, Wang J, Ho MY, Brudvig GW, Bryant DA
OpenAccess logo J Biol Chem 298 101408 (2022)
PMID: 34793839
Related structures: EMD-24821

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-110144 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chains: A, G, a
Molecule details ›
Chains: A, G, a
Length: 782 amino acids
Theoretical weight: 86.41 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP20 (Residues: 1-782; Coverage: 100%)
Gene names: S7335_5506, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chains: B, H, b
Molecule details ›
Chains: B, H, b
Length: 743 amino acids
Theoretical weight: 83.21 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP21 (Residues: 1-743; Coverage: 100%)
Gene names: S7335_4329, psaB
Sequence domains: Photosystem I psaA/psaB protein
PsaC Chains: C, N, c
Molecule details ›
Chains: C, N, c
Length: 81 amino acids
Theoretical weight: 8.81 KDa
Source organism: Synechococcus sp. PCC 7335
Photosystem I reaction center subunit II Chains: D, O, d
Molecule details ›
Chains: D, O, d
Length: 155 amino acids
Theoretical weight: 17.05 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WFP8 (Residues: 1-155; Coverage: 100%)
Gene name: S7335_2287
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chains: E, P, e
Molecule details ›
Chains: E, P, e
Length: 71 amino acids
Theoretical weight: 7.96 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WSJ5 (Residues: 1-71; Coverage: 100%)
Gene names: S7335_5210, psaE
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chains: F, Q, f
Molecule details ›
Chains: F, Q, f
Length: 168 amino acids
Theoretical weight: 18.57 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP24 (Residues: 1-168; Coverage: 100%)
Gene name: S7335_5139
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit VIII Chains: I, R, i
Molecule details ›
Chains: I, R, i
Length: 70 amino acids
Theoretical weight: 7.67 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP23 (Residues: 1-70; Coverage: 100%)
Gene name: S7335_2282
Photosystem I reaction center subunit IX Chains: J, S, j
Molecule details ›
Chains: J, S, j
Length: 46 amino acids
Theoretical weight: 5.17 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP25 (Residues: 1-46; Coverage: 100%)
Gene name: S7335_5198
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Photosystem I reaction center subunit PsaK Chains: K, T, k
Molecule details ›
Chains: K, T, k
Length: 84 amino acids
Theoretical weight: 8.2 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WL17 (Residues: 1-84; Coverage: 100%)
Gene names: S7335_5488, psaK
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit XI Chains: L, U, l
Molecule details ›
Chains: L, U, l
Length: 174 amino acids
Theoretical weight: 18.63 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WP22 (Residues: 1-174; Coverage: 100%)
Gene name: S7335_2771
Sequence domains: Photosystem I reaction centre subunit XI
PsaM Chains: M, V, m
Molecule details ›
Chains: M, V, m
Length: 31 amino acids
Theoretical weight: 3.37 KDa
Source organism: Synechococcus sp. PCC 7335
2Fe-2S ferredoxin-type domain-containing protein Chains: W, X, x
Molecule details ›
Chains: W, X, x
Length: 99 amino acids
Theoretical weight: 10.84 KDa
Source organism: Synechococcus sp. PCC 7335
UniProt:
  • Canonical: B4WFX2 (Residues: 1-99; Coverage: 100%)
Gene name: S7335_2822
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain

Ligands and Environments

12 bound ligands:
Ligand CL0 3 x CL0
Ligand CLA 255 x CLA
Ligand F6C 18 x F6C
Ligand PQN 6 x PQN
Ligand SF4 9 x SF4
Ligand BCR 57 x BCR
Ligand LHG 12 x LHG
Ligand LMG 9 x LMG
Ligand LMT 39 x LMT
Ligand CL 3 x CL
Ligand CA 3 x CA
Ligand FES 3 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.91Å
Relevant EMDB volumes: EMD-24821

Quick links

Citations

1 review citation

Microbial applications for sustainable space exploration beyond low Earth orbit.
Koehle et al. (2023)
8 other articles

3 mentions without citation

Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation.
Gisriel et al. (2022)
2 more