7s3h

Electron Microscopy
2.5Å resolution

Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation

Released:
Primary publication:
CRISPR-Cas9 bends and twists DNA to read its sequence.
OpenAccess logo Nat Struct Mol Biol 29 395-402 (2022)
PMID: 35422516
Related structures: EMD-24823

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-121942 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: P
Molecule details ›
Chain: P
Length: 1371 amino acids
Theoretical weight: 158.97 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Non-target DNA strand Chain: N
Molecule details ›
Chain: N
Length: 30 nucleotides
Theoretical weight: 9.31 KDa
Source organism: synthetic construct
Expression system: Not provided
Target DNA strand Chain: T
Molecule details ›
Chain: T
Length: 30 nucleotides
Theoretical weight: 9.19 KDa
Source organism: synthetic construct
Expression system: Not provided
Single-guide RNA Chain: R
Molecule details ›
Chain: R
Length: 102 nucleotides
Theoretical weight: 32.79 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-24823
Expression systems:
  • Escherichia coli
  • Not provided