› 7s4o

X-ray diffraction
1.4Å resolution

Structure of C208A Sortase A from Streptococcus pyogenes bound to LPATS peptide

Released:
DOI: 10.2210/pdb7s4o/pdb
PDB entry 7s4o coloured by chain, front view.
PDB entry 7s4o coloured by chain, side view.
PDB entry 7s4o coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Streptococcus pyogenes Class A sortase in complex with substrate and product mimics provide key details of target recognition.
Johnson DA, Piper IM, Vogel BA, Jackson SN, Svendsen JE, Kodama HM, Lee DE, Lindblom KM, McCarty J, Antos JM, Amacher JF
OpenAccess logo J Biol Chem 102446 (2022)
PMID: 36055407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sortase Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 18.59 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZN4 (Residues: 81-249; Coverage: 68%)
Gene name: SPy_1154
Sequence domains: Sortase domain
LEU-PRO-ALA-THR-SER-GLY Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 880 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand BE2 2 x BE2
Ligand DNP 2 x DNP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 38.489Å b: 59.098Å c: 64.574Å
α: 90° β: 101.69° γ: 90°
R-values:
R R work R free
0.17 0.169 0.194
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Substrate-derived Sortase A inhibitors: targeting an essential virulence factor of Gram-positive pathogenic bacteria.
Abujubara et al. (2023)

1 mention without citation

A unique binding mode of P1' Leu-containing target sequences for Streptococcus pyogenes sortase A results in alternative cleavage.
Vogel et al. (2024)