› 7s57

X-ray diffraction
1.7Å resolution

Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A

Released:
DOI: 10.2210/pdb7s57/pdb
PDB entry 7s57 coloured by chain, front view.
PDB entry 7s57 coloured by chain, side view.
PDB entry 7s57 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes.
Gao M, Johnson DA, Piper IM, Kodama HM, Svendsen JE, Tahti E, Longshore-Neate F, Vogel B, Antos JM, Amacher JF
Protein Sci 31 701-715 (2022)
PMID: 34939250

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sortase Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.55 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZN4 (Residues: 81-249; Coverage: 68%)
Gene name: SPy_1154
Sequence domains: Sortase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 33.596Å b: 56.917Å c: 71.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Structures of Streptococcus pyogenes class A sortase in complex with substrate and product mimics provide key details of target recognition.
Johnson et al. (2022)
1 more

1 mention without citation

Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes.
Gao et al. (2022)