› 7s5u

X-ray diffraction
4.41Å resolution

Extended bipolar assembly domain of kinesin-5 minifilament

Released:
DOI: 10.2210/pdb7s5u/pdb
PDB entry 7s5u coloured by chain, front view.
PDB entry 7s5u coloured by chain, side view.
PDB entry 7s5u coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The kinesin-5 tail and bipolar miniflament domains are the origin of its microtubule crosslinking and sliding activity.
Nithianantham S, Iwanski MK, Gaska I, Pandey H, Bodrug T, Inagaki S, Major J, Brouhard GJ, Gheber L, Rosenfeld SS, Forth S, Hendricks AG, Al-Bassam J
OpenAccess logo Mol Biol Cell mbcE23070287 (2023)
PMID: 37610838

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein Klp61F Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 28.43 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46863 (Residues: 597-835; Coverage: 22%)
Gene names: CG9191, KLP2, Klp61F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 253.18Å b: 84.89Å c: 96.77Å
α: 90° β: 91.44° γ: 90°
R-values:
R R work R free
0.277 0.274 0.31
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

The kinesin-5 tail and bipolar minifilament domains are the origin of its microtubule crosslinking and sliding activity.
Nithianantham et al. (2023)