› 7s65

Electron Microscopy
3.2Å resolution

Compressed conformation of nighttime state KaiC

Released:
DOI: 10.2210/pdb7s65/pdb
PDB entry 7s65 coloured by chain, front view.
PDB entry 7s65 coloured by chain, side view.
PDB entry 7s65 coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
Coupling of distant ATPase domains in the circadian clock protein KaiC.
Swan JA, Sandate CR, Chavan AG, Freeberg AM, Etwaru D, Ernst DC, Palacios JG, Golden SS, LiWang A, Lander GC, Partch CL
Nat Struct Mol Biol (2022)
PMID: 35864165
Related structures: EMD-24850

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 519 amino acids
Theoretical weight: 58.08 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC

Ligands and Environments

3 bound ligands:
Ligand MG 12 x MG
Ligand ADP 2 x ADP
Ligand ATP 10 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-24850
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

The inner workings of an ancient biological clock.
Fang et al. (2024)
3 other articles

2 mentions without citation

Coupling of distant ATPase domains in the circadian clock protein KaiC.
Swan et al. (2022)
1 more