› 7s6s

X-ray diffraction
1.98Å resolution

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit DBL1

Released:
DOI: 10.2210/pdb7s6s/pdb
PDB entry 7s6s coloured by chain, front view.
PDB entry 7s6s coloured by chain, side view.
PDB entry 7s6s coloured by chain, top view.
Source organism: Methylosinus trichosporium OB3b
Primary publication:
X-ray Crystal Structures of Methane Monooxygenase Hydroxylase Complexes with Variants of Its Regulatory Component: Correlations with Altered Reaction Cycle Dynamics.
Jones JC, Banerjee R, Semonis MM, Shi K, Aihara H, Lipscomb JD
Biochemistry 61 21-33 (2022)
PMID: 34910460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-104201 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methane monooxygenase component A alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 515 amino acids
Theoretical weight: 58.97 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D2D5X0 (Residues: 12-526; Coverage: 98%)
Gene name: CQW49_12480
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chains: B, F
Molecule details ›
Chains: B, F
Length: 392 amino acids
Theoretical weight: 44.95 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D2D5X7 (Residues: 4-395; Coverage: 99%)
Gene name: CQW49_12475
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chains: C, G
Molecule details ›
Chains: C, G
Length: 168 amino acids
Theoretical weight: 19.31 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D2D0T0 (Residues: 2-169; Coverage: 99%)
Gene name: CQW49_12465
Sequence domains: Methane monooxygenase, hydrolase gamma chain
Methane monooxygenase regulatory protein B Chains: D, H
Molecule details ›
Chains: D, H
Length: 136 amino acids
Theoretical weight: 14.61 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D2D0T8 (Residues: 3-138; Coverage: 99%)
Gene name: CQW49_12470
Sequence domains: MmoB/DmpM family

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand EDO 50 x EDO
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 102.73Å b: 105.71Å c: 299.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.188
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

X-ray Crystal Structures of Methane Monooxygenase Hydroxylase Complexes with Variants of Its Regulatory Component: Correlations with Altered Reaction Cycle Dynamics.
Jones et al. (2022)