› 7s80

X-ray diffraction
1.4Å resolution

Crystal structure of iAChSnFR Acetylcholine Sensor precursor binding protein

Released:
DOI: 10.2210/pdb7s80/pdb
PDB entry 7s80 coloured by chain, front view.
PDB entry 7s80 coloured by chain, side view.
PDB entry 7s80 coloured by chain, top view.
Source organism: Thermoanaerobacter sp. X513
Primary publication:
Structure, Function, and Application of Bacterial ABC Transporters
Fan C
Ph.D.Thesis,California Institute of Technology (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
iAchSnFR Fluorescent Acetylcholine Sensor precursor binding protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33 KDa
Source organism: Thermoanaerobacter sp. X513
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand MLI 1 x MLI
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 41.536Å b: 60.816Å c: 58.657Å
α: 90° β: 100.519° γ: 90°
R-values:
R R work R free
0.17 0.17 0.195
Expression system: Escherichia coli BL21(DE3)

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