7s8l

Electron Microscopy
2.45Å resolution

CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208008 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Mas-related G-protein coupled receptor member X2 Chain: R
Molecule details ›
Chain: R
Length: 331 amino acids
Theoretical weight: 37.15 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96LB1 (Residues: 2-330; Coverage: 100%)
Gene names: MRGPRX2, MRGX2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Cortistatin-17 Chain: A
Molecule details ›
Chain: A
Length: 14 amino acids
Theoretical weight: 1.73 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O00230 (Residues: 92-105; Coverage: 16%)
Gene names: CORT, UNQ307/PRO350
Sequence domains: Somatostatin/Cortistatin family
Gs-mini-Gq chimera Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 257 amino acids
Theoretical weight: 27.41 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.45Å
Relevant EMDB volumes: EMD-24896
Expression systems:
  • Trichoplusia ni
  • Not provided
  • Spodoptera frugiperda