› 7s8m

Electron Microscopy
2.54Å resolution

CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14

Released:
DOI: 10.2210/pdb7s8m/pdb
PDB entry 7s8m coloured by chain, front view.
PDB entry 7s8m coloured by chain, side view.
PDB entry 7s8m coloured by chain, top view.
Source organisms:
Primary publication:
Structure, function and pharmacology of human itch GPCRs.
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature 600 170-175 (2021)
PMID: 34789874
Related structures: EMD-24897

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208006 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
scFv16 Chain: E
Mas-related G-protein coupled receptor member X2; Soluble cytochrome b562 Chain: R
Cortistatin-17 Chain: A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-24897
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

20 review citations

Bile acids and the gut microbiota: metabolic interactions and impacts on disease.
Collins et al. (2023)
19 more

5 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
4 more

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 7s8m found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-10855
Datasets: 1
Total size: 2800Gb
EMPIAR thumbnail