7s8p

Electron Microscopy
2.6Å resolution

CryoEM structure of Gq-coupled MRGPRX4 with small molecule agonist MS47134

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209567 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Gs-mini-Gq chimera Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Mas-related G-protein coupled receptor member X4; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 472 amino acids
Theoretical weight: 53.42 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q96LA9 (Residues: 2-322; Coverage: 100%)
Gene names: MRGPRX4, MRGX4, SNSR5, SNSR6, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-24900
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda