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Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes.

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Hennell James R, Deme JC, Hunter A, Berks BC, Lea SM
OpenAccess logo mBio e0026722 (2022)
Related entries: 7sat, 7sau, 7saz, 7sb2

Cited: 7 times
EuropePMC logo PMID: 35446127

Abstract

Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are energized by the GldLM motor complex, which transduces the proton motive force at the inner membrane into mechanical work at the outer membrane. We previously used cryo-electron microscopy to solve the structure of the GldLM motor core from Flavobacterium johnsoniae at 3.9-Å resolution (R. Hennell James, J. C. Deme, A. Kjaer, F. Alcock, et al., Nat Microbiol 6:221-233, 2021, https://dx.doi.org/10.1038/s41564-020-00823-6). Here, we present structures of homologous complexes from a range of pathogenic and environmental Bacteroidetes species at up to 3.0-Å resolution. These structures show that the architecture of the GldLM motor core is conserved across the Bacteroidetes phylum, although there are species-specific differences at the N terminus of GldL. The resolution improvements reveal a cage-like structure that ties together the membrane-proximal cytoplasmic region of GldL and influences gliding function. These findings add detail to our structural understanding of bacterial ion-driven motors that drive the T9SS and gliding motility.

Articles - 7sax mentioned but not cited (1)

  1. Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes. Hennell James R, Deme JC, Hunter A, Berks BC, Lea SM. mBio 13 e0026722 (2022)


Articles citing this publication (6)

  1. A unique bacterial secretion machinery with multiple secretion centers. Song L, Perpich JD, Wu C, Doan T, Nowakowska Z, Potempa J, Christie PJ, Cascales E, Lamont RJ, Hu B. Proc Natl Acad Sci U S A 119 e2119907119 (2022)
  2. Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery. Shibata S, Tahara YO, Katayama E, Kawamoto A, Kato T, Zhu Y, Nakane D, Namba K, Miyata M, McBride MJ, Nakayama K. Commun Biol 6 94 (2023)
  3. A journey with type IX secretion system effectors: selection, transport, processing and activities. Paillat M, Lunar Silva I, Cascales E, Doan T. Microbiology (Reading) 169 (2023)
  4. Structural insights into the mechanism of protein transport by the Type 9 Secretion System translocon. Lauber F, Deme JC, Liu X, Kjær A, Miller HL, Alcock F, Lea SM, Berks BC. Nat Microbiol 9 1089-1102 (2024)
  5. Structure-function analysis of PorXFj, the PorX homolog from Flavobacterium johnsioniae, suggests a role of the CheY-like domain in type IX secretion motor activity. Zammit M, Bartoli J, Kellenberger C, Melani P, Roussel A, Cascales E, Leone P. Sci Rep 14 6577 (2024)
  6. T9GPred: A Comprehensive Computational Tool for the Prediction of Type 9 Secretion System, Gliding Motility, and the Associated Secreted Proteins. Sahoo AK, Vivek-Ananth RP, Chivukula N, Rajaram SV, Mohanraj K, Khare D, Acharya C, Samal A. ACS Omega 8 34091-34102 (2023)


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