› 7scq

Electron Microscopy
6Å resolution

tRNA-like Structure from Brome Mosaic Virus Bound to Tyrosyl-tRNA Synthetase from Phaseolus vulgaris. Conformation: Bound State 2.

Released:
DOI: 10.2210/pdb7scq/pdb
PDB entry 7scq coloured by chain, front view.
PDB entry 7scq coloured by chain, side view.
PDB entry 7scq coloured by chain, top view.
Source organisms:
Primary publication:
A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure.
Bonilla SL, Sherlock ME, MacFadden A, Kieft JS
Science 374 955-960 (2021)
PMID: 34793227
Related structures: EMD-25041

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250477 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 45.48 KDa
Source organism: Phaseolus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: V7CJ18 (Residues: 1-379; Coverage: 100%)
Gene name: PHAVU_002G027700g
Sequence domains: tRNA synthetases class I (W and Y)
tRNA-like structure from brome mosaic virus RNA 3. Chain: C
Molecule details ›
Chain: C
Length: 171 nucleotides
Theoretical weight: 55.04 KDa
Source organism: Brome mosaic virus
Expression system: Not provided
Sequence domains: Pseudoknot of tRNA-like structure

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6Å
Relevant EMDB volumes: EMD-25041
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Cryo-EM advances in RNA structure determination.
Ma et al. (2022)
10 more

2 mentions without citation

The promise of cryo-EM to explore RNA structural dynamics.
Bonilla et al. (2022)
1 more