› 7sf7

Electron Microscopy
2.9Å resolution

LPHN3 (ADGRL3) 7TM domain bound to tethered agonist in complex with G protein heterotrimer

Released:
DOI: 10.2210/pdb7sf7/pdb
PDB entry 7sf7 coloured by chain, front view.
PDB entry 7sf7 coloured by chain, side view.
PDB entry 7sf7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The tethered peptide activation mechanism of adhesion GPCRs.
Barros-Álvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature 604 757-762 (2022)
PMID: 35418682
Related structures: EMD-25076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-276231 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Adhesion G protein-coupled receptor L3 Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 34.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HAR2 (Residues: 842-1138; Coverage: 21%)
Gene names: ADGRL3, KIAA0768, LEC3, LPHN3
Sequence domains:
Guanine nucleotide-binding protein subunit alpha-13 Chain: B
Molecule details ›
Chain: B
Length: 230 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q14344 (Residues: 17-377; Coverage: 61%)
Gene name: GNA13
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-25076
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

The GPCR properties of polycystin-1- A new paradigm.
Maser et al. (2022)
7 more

5 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
4 more