› 7sgd

Electron Microscopy
3.97Å resolution

Lassa virus glycoprotein construct(Josiah GPCysR4) recovered from GPC-I53-50 nanoparticle by localized reconstruction

Released:
DOI: 10.2210/pdb7sgd/pdb
PDB entry 7sgd coloured by chain, front view.
PDB entry 7sgd coloured by chain, side view.
PDB entry 7sgd coloured by chain, top view.
Source organism: Lassa mammarenavirus
Primary publication:
Lassa virus glycoprotein nanoparticles elicit neutralizing antibody responses and protection.
Brouwer PJM, Antanasijevic A, Ronk AJ, Müller-Kräuter H, Watanabe Y, Claireaux M, Perrett HR, Bijl TPL, Grobben M, Umotoy JC, Schriek AI, Burger JA, Tejjani K, Lloyd NM, Steijaert TH, van Haaren MM, Sliepen K, de Taeye SW, van Gils MJ, Crispin M, Strecker T, Bukreyev A, Ward AB, Sanders RW
OpenAccess logo Cell Host Microbe (2022)
PMID: 36400021
Related structures: EMD-25107

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Pre-glycoprotein polyprotein GP complex Chains: A, B, C, a, b, c
Molecule details ›
Chains: A, B, C, a, b, c
Length: 665 amino acids
Theoretical weight: 73.85 KDa
Source organism: Lassa mammarenavirus
Expression system: Homo sapiens
UniProt:
  • Canonical: P08669 (Residues: 1-424; Coverage: 86%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 36 x NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Ligand MAN 6 x MAN
Carbohydrate polymer : NEW Components: NAG, FUC
Ligand NAG 6 x NAG
Ligand FUC 3 x FUC
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.97Å
Relevant EMDB volumes: EMD-25107
Expression system: Homo sapiens

Quick links

Citations

6 citation in other articles

A Lassa virus mRNA vaccine confers protection but does not require neutralizing antibody in a guinea pig model of infection.
Ronk et al. (2023)
5 more

3 mentions without citation

Lassa virus glycoprotein nanoparticles elicit neutralizing antibody responses and protection.
Brouwer et al. (2022)
2 more