› 7shi

X-ray diffraction
2Å resolution

Crystal Structure of Cytochrome P450 AmphL from Streptomyces nodosus and the Structural Basis for Substrate Selectivity in Macrolide Metabolizing P450s

Released:
DOI: 10.2210/pdb7shi/pdb
PDB entry 7shi coloured by chain, front view.
PDB entry 7shi coloured by chain, side view.
PDB entry 7shi coloured by chain, top view.
Source organism: Streptomyces nodosus
Primary publication:
Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s.
Amaya JA, Lamb DC, Kelly SL, Caffrey P, Murarka VC, Poulos TL
OpenAccess logo J Biol Chem 298 101746 (2022)
PMID: 35189143

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AmphL cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.18 KDa
Source organism: Streptomyces nodosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93NX6 (Residues: 1-396; Coverage: 100%)
Gene names: CP978_03045, SNOD_02645, amphL
Sequence domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand 4UH 2 x 4UH
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 73.458Å b: 68.154Å c: 147.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining.
Caffrey et al. (2022)

2 mentions without citation

Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s.
Amaya et al. (2022)
1 more