› 7skg

X-ray diffraction
1.33Å resolution

Myocilin OLF mutant V329M

Released:
DOI: 10.2210/pdb7skg/pdb
PDB entry 7skg coloured by chain, front view.
PDB entry 7skg coloured by chain, side view.
PDB entry 7skg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability.
Scelsi HF, Hill KR, Barlow BM, Martin MD, Lieberman RL
OpenAccess logo Dis Model Mech (2022)
PMID: 36579626

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myocilin, C-terminal fragment Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99972 (Residues: 228-504; Coverage: 59%)
Gene names: GLC1A, MYOC, TIGR
Sequence domains: Olfactomedin-like domain

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand CA 1 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 49.479Å b: 50.73Å c: 50.508Å
α: 90° β: 96.22° γ: 90°
R-values:
R R work R free
0.167 0.167 0.183
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Myocilin misfolding and glaucoma: A 20-year update.
Saccuzzo et al. (2023)
1 other article

1 mention without citation

Quantitative differentiation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability.
Scelsi et al. (2023)