› 7skw

Electron crystallography
0.87Å resolution

Ab initio structure of triclinic lysozyme from electron-counted MicroED data

Released:
DOI: 10.2210/pdb7skw/pdb
PDB entry 7skw coloured by chain, front view.
PDB entry 7skw coloured by chain, side view.
PDB entry 7skw coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Ab initio phasing macromolecular structures using electron-counted MicroED data.
Martynowycz MW, Clabbers MTB, Hattne J, Gonen T
OpenAccess logo Nat Methods 19 724-729 (2022)
PMID: 35637302
Related structures: EMD-25184

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

1 bound ligand:
Ligand NO3 4 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 0.87Å
Relevant EMDB volumes: EMD-25184

Quick links

Citations

5 review citations

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
Chua et al. (2022)
4 more

3 mentions without citation

Protein Data Bank: A Comprehensive Review of 3D Structure Holdings and Worldwide Utilization by Researchers, Educators, and Students.
Burley et al. (2022)
2 more