› 7so7

X-ray diffraction
3.59Å resolution

Novel structural insights for a pair of monoclonal antibodies recognizing non-overlapping epitopes of the glucosyltransferase domain of Clostridium difficile toxin B

Released:
DOI: 10.2210/pdb7so7/pdb
PDB entry 7so7 coloured by chain, front view.
PDB entry 7so7 coloured by chain, side view.
PDB entry 7so7 coloured by chain, top view.
Source organisms:
Primary publication:
Novel structural insights for a pair of monoclonal antibodies recognizing non-overlapping epitopes of the glucosyltransferase domain of Clostridium difficile toxin B.
Liu J, Kothe M, Zhang J, Oloo E, Stegalkina S, Mundle ST, Li L, Zhang J, Cole LE, Barone L, Biemann HP, Kleanthous H, Anosova NG, Anderson SF
OpenAccess logo Curr Res Struct Biol 4 96-105 (2022)
PMID: 35469152

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210732 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glucosyltransferase TcdB Chains: A, B
Molecule details ›
Chains: A, B
Length: 538 amino acids
Theoretical weight: 62.65 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P18177 (Residues: 1-538; Coverage: 23%)
Gene names: tcdB, toxB
Sequence domains:
Fab B1 HC Chains: H, X
Molecule details ›
Chains: H, X
Length: 213 amino acids
Theoretical weight: 22.8 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
FAB B1 LC Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 218 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 210.54Å b: 320.08Å c: 65.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.258
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

1 citations in other articles

Fit-for-purpose heterodivalent single-domain antibody for gastrointestinal targeting of toxin B from Clostridium difficile.
Rodriguez Rodriguez et al. (2024)

1 mention without citation

Novel structural insights for a pair of monoclonal antibodies recognizing non-overlapping epitopes of the glucosyltransferase domain of Clostridium difficile toxin B.
Liu et al. (2022)