› 7sq0

Electron Microscopy
3.7Å resolution

Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1

Released:
DOI: 10.2210/pdb7sq0/pdb
PDB entry 7sq0 coloured by chain, front view.
PDB entry 7sq0 coloured by chain, side view.
PDB entry 7sq0 coloured by chain, top view.
Source organisms:
Primary publication:
Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.
Fry MY, Najdrová V, Maggiolo AO, Saladi SM, Doležal P, Clemons WM
Nat Struct Mol Biol (2022)
PMID: 35851188
Related structures: EMD-25373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108576 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase ASNA1 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 39.16 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8B3G9 (Residues: 1-354; Coverage: 100%)
Gene name: GL50803_7953
Sequence domains: Anion-transporting ATPase
Protein transport protein BOS1; Soluble cytochrome b562 Chain: C
Molecule details ›
Chain: C
Length: 139 amino acids
Theoretical weight: 15.86 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 22-127; Coverage: 99%)
  • Canonical: P25385 (Residues: 222-240; Coverage: 8%)
Gene names: BOS1, L9449.9, YLR078C, cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 1 x ZN
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-25373
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Role of Hsp70 in Post-Translational Protein Targeting: Tail-Anchored Membrane Proteins and Beyond.
Shan SO. (2023)
2 other articles

1 mention without citation

Structures of Get3d reveal a distinct architecture associated with the emergence of photosynthesis.
Barlow et al. (2023)