› 7sqk

Electron Microscopy
8Å resolution

Cryo-EM structure of the human augmin complex

Released:
DOI: 10.2210/pdb7sqk/pdb
PDB entry 7sqk coloured by chain, front view.
PDB entry 7sqk coloured by chain, side view.
PDB entry 7sqk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular architecture of the augmin complex.
Gabel CA, Li Z, DeMarco AG, Zhang Z, Yang J, Hall MC, Barford D, Chang L
OpenAccess logo Nat Commun 13 5449 (2022)
PMID: 36114186
Related structures: EMD-25387

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131798 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
HAUS augmin-like complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96CS2 (Residues: 1-278; Coverage: 100%)
Gene names: CCDC5, HAUS1, HEIC
HAUS augmin-like complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 284 amino acids
Theoretical weight: 32.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NVX0 (Residues: 1-235; Coverage: 100%)
Gene names: C15orf25, CEP27, HAUS2
Sequence domains: HAUS augmin-like complex subunit 2
HAUS augmin-like complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 603 amino acids
Theoretical weight: 69.74 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q68CZ6 (Residues: 1-603; Coverage: 100%)
Gene names: C4orf15, HAUS3
Sequence domains: HAUS augmin-like complex subunit 3
HAUS augmin-like complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 318 amino acids
Theoretical weight: 37.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H6D7 (Residues: 1-363; Coverage: 88%)
  • Best match: Q9H6D7-4 (Residues: 1-318)
Gene names: C14orf94, HAUS4
Sequence domains: HAUS augmin-like complex subunit 4
HAUS augmin-like complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 633 amino acids
Theoretical weight: 71.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O94927 (Residues: 1-633; Coverage: 100%)
Gene names: HAUS5, KIAA0841
Sequence domains: HAUS augmin-like complex subunit 5
HAUS augmin-like complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 432 amino acids
Theoretical weight: 50.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7Z4H7 (Residues: 1-432; Coverage: 45%)
Gene names: DGT6, FAM29A, HAUS6, KIAA1574
Sequence domains: HAUS augmin-like complex subunit 6 N-terminus
HAUS augmin-like complex subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 368 amino acids
Theoretical weight: 40.82 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q99871 (Residues: 1-358; Coverage: 100%)
Gene names: HAUS7, UCHL5IP, UIP1
HAUS augmin-like complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 410 amino acids
Theoretical weight: 44.92 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BT25 (Residues: 1-410; Coverage: 100%)
Gene names: HAUS8, HICE1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-25387
Expression system: Trichoplusia ni

Quick links

Citations

2 citation in other articles

Augmin is a Ran-regulated spindle assembly factor.
Kraus et al. (2023)
1 more

2 mentions without citation

Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.
Ustinova et al. (2023)
1 more