7srr

Electron Microscopy
2.9Å resolution

5-HT2B receptor bound to LSD in complex with heterotrimeric mini-Gq protein obtained by cryo-electron microscopy (cryoEM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211201 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
G protein subunit q (Gi2-mini-Gq chimera) Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
single-chain variable fragment 16 (scFv16) Chain: E
Molecule details ›
Chain: E
Length: 286 amino acids
Theoretical weight: 30.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
5-hydroxytryptamine receptor 2B Chain: R
Molecule details ›
Chain: R
Length: 370 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41595 (Residues: 36-405; Coverage: 77%)
Gene name: HTR2B
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-25402
Expression system: Spodoptera frugiperda