› 7ssz

Electron Microscopy
3.25Å resolution

Structure of human Kv1.3 with A0194009G09 nanobodies

Released:
DOI: 10.2210/pdb7ssz/pdb
PDB entry 7ssz coloured by chain, front view.
PDB entry 7ssz coloured by chain, side view.
PDB entry 7ssz coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators.
Selvakumar P, Fernández-Mariño AI, Khanra N, He C, Paquette AJ, Wang B, Huang R, Smider VV, Rice WJ, Swartz KJ, Meyerson JR
OpenAccess logo Nat Commun 13 3854 (2022)
PMID: 35788586
Related structures: EMD-25417

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238729 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein; Potassium voltage-gated channel subfamily A member 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 856 amino acids
Theoretical weight: 95.02 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P22001 (Residues: 1-575; Coverage: 100%)
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene names: GFP, HGK5, KCNA3
Sequence domains:
Nanobody A0194009G09 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 126 amino acids
Theoretical weight: 13.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-25417
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

1 review citation

Microglial ion channels: Key players in non-cell autonomous neurodegeneration.
Sarkar S. (2022)
11 other articles

1 mention without citation

Structure of the voltage-gated potassium channel KV1.3: Insights into the inactivated conformation and binding to therapeutic leads.
Chandy et al. (2023)