› 7st8

X-ray diffraction
2.75Å resolution

Crystal structure of 7H2.2 Fab in complex with SAS1B C-terminal region

Released:
DOI: 10.2210/pdb7st8/pdb
PDB entry 7st8 coloured by chain, front view.
PDB entry 7st8 coloured by chain, side view.
PDB entry 7st8 coloured by chain, top view.
Source organisms:
Primary publication:
Monoclonal antibody 7H2.2 binds the C-terminus of the cancer-oocyte antigen SAS1B through the hydrophilic face of a conserved amphipathic helix corresponding to one of only two regions predicted to be ordered.
Legg MSG, Gagnon SML, Powell CJ, Boulanger MJ, Li AJJ, Evans SV
Acta Crystallogr D Struct Biol 78 623-632 (2022)
PMID: 35503210

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212372 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
7H2.2 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Mus musculus
7H2.2 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.85 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Astacin-like metalloendopeptidase Chain: S
Molecule details ›
Chain: S
Length: 170 amino acids
Theoretical weight: 17.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HA08 (Residues: 284-431; Coverage: 36%)
Gene name: ASTL

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 132.355Å b: 43.03Å c: 106.227Å
α: 90° β: 123.89° γ: 90°
R-values:
R R work R free
0.233 0.231 0.27
Expression systems:
  • Mus musculus
  • Escherichia coli

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