7stf

Electron Microscopy
3.14Å resolution

Structure of KRAS G12V/HLA-A*03:01 in complex with antibody fragment V2

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-233891 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
IgG, Fab Heavy Chain V2 Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.18 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector BsrGI-MCS-pcDNA3.1
GTPase KRas, N-terminally processed Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 885 Da
Source organism: synthetic construct
Expression system: Not provided
IgG, Fab Light Chain V2 Chain: L
HLA class I histocompatibility antigen, A alpha chain Chain: A
Beta-2-microglobulin Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.14Å
Relevant EMDB volumes: EMD-25427
Expression systems:
  • Mammalian expression vector BsrGI-MCS-pcDNA3.1
  • Not provided
  • Escherichia coli BL21(DE3)