› 7str

X-ray diffraction
1.5Å resolution

Crystal Structure of Human Fab S24-1063 in the Complex with the N-teminal Domain of Nucleocapsid Protein from SARS CoV-2

Released:
DOI: 10.2210/pdb7str/pdb
PDB entry 7str coloured by chain, front view.
PDB entry 7str coloured by chain, side view.
PDB entry 7str coloured by chain, top view.
Source organisms:
Primary publication:
Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human monoclonal antibodies.
Kim Y, Maltseva N, Tesar C, Jedrzejczak R, Endres M, Ma H, Dugan HL, Stamper CT, Chang C, Li L, Changrob S, Zheng NY, Huang M, Ramanathan A, Wilson P, Michalska K, Joachimiak A
OpenAccess logo iScience 27 108976 (2024)
PMID: 38327783

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276140 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab S24-1063, Light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab S24-1063, Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Nucleoprotein Chain: C
Molecule details ›
Chain: C
Length: 130 amino acids
Theoretical weight: 14.3 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTC9 (Residues: 47-173; Coverage: 30%)
Gene name: N
Sequence domains: Coronavirus nucleocapsid

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 54.021Å b: 67.134Å c: 84.459Å
α: 90° β: 107.022° γ: 90°
R-values:
R R work R free
0.163 0.161 0.202
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human monoclonal antibodies.
Kim et al. (2024)