› 7sus

X-ray diffraction
2.7Å resolution

Crystal structure of Apelin receptor in complex with small molecule

Released:
DOI: 10.2210/pdb7sus/pdb
PDB entry 7sus coloured by chain, front view.
PDB entry 7sus coloured by chain, side view.
PDB entry 7sus coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into apelin receptor-G protein stoichiometry.
Yue Y, Liu L, Wu LJ, Wu Y, Wang L, Li F, Liu J, Han GW, Chen B, Lin X, Brouillette RL, Breault É, Longpré JM, Shi S, Lei H, Sarret P, Stevens RC, Hanson MA, Xu F
Nat Struct Mol Biol 29 688-697 (2022)
PMID: 35817871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apelin receptor; Rubredoxin Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 46.44 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35414 (Residues: 7-229, 243-330; Coverage: 82%)
  • Canonical: P00268 (Residues: 1-54; Coverage: 100%)
Gene names: AGTRL1, APJ, APLNR
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand 8EH 1 x 8EH
Ligand OLC 1 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 163.01Å b: 44.56Å c: 79.68Å
α: 90° β: 115.62° γ: 90°
R-values:
R R work R free
0.253 0.252 0.274
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
1 more