› 7sva

Electron Microscopy
3.26Å resolution

Cryo-EM structure of Arabidopsis Ago10-guide RNA complex

Released:
DOI: 10.2210/pdb7sva/pdb
PDB entry 7sva coloured by chain, front view.
PDB entry 7sva coloured by chain, side view.
PDB entry 7sva coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for RNA slicing by a plant Argonaute.
Xiao Y, Maeda S, Otomo T, MacRae IJ
Nat Struct Mol Biol (2023)
PMID: 37127820
Related structures: EMD-25446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216066 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute 10 Chain: A
Molecule details ›
Chain: A
Length: 988 amino acids
Theoretical weight: 110.98 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9XGW1 (Residues: 1-988; Coverage: 100%)
Gene names: AGO10, At5g43810, MQD19.17, PNH, ZLL
Sequence domains:
RNA (5'-R(P*UP*GP*GP*AP*GP*UP*GP*UP*GP*AP*CP*AP*AP*UP*GP*GP*UP*GP*UP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-25446
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

2 review citations

When Argonaute takes out the ribonuclease sword.
Nakanishi K. (2024)
1 more

2 mentions without citation

Structural basis for RNA slicing by a plant Argonaute.
Xiao et al. (2023)
1 more