› 7sxc

Solution NMR

cTnC-TnI chimera complexed with calcium

Released:
DOI: 10.2210/pdb7sxc/pdb
PDB entry 7sxc coloured by chain, ensemble of 10 models, front view.
PDB entry 7sxc coloured by chain, ensemble of 10 models, side view.
PDB entry 7sxc coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Thermodynamic Model for the Activation of Cardiac Troponin.
Poppe L, Hartman JJ, Romero A, Reagan JD
Biochemistry 61 741-748 (2022)
PMID: 35349258

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles; Troponin I, cardiac muscle Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63316 (Residues: 2-91; Coverage: 56%)
  • Canonical: P19429 (Residues: 147-180; Coverage: 16%)
Gene names: TNNC, TNNC1, TNNI3
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 95%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays.
Parijat et al. (2023)