7syv

Electron Microscopy
3.9Å resolution

Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt)

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 39-mer (preferred)
PDBe Complex ID:
PDB-CPX-239233 (preferred)
Entry contents:
36 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (39 distinct):
40S ribosomal protein S24 Chain: Z
Molecule details ›
Chain: Z
Length: 131 amino acids
Theoretical weight: 15.24 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T3D8 (Residues: 1-131; Coverage: 100%)
Gene name: RPS24
Sequence domains: Ribosomal protein S24e
Eukaryotic translation initiation factor 1A, X-chromosomal Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P47813 (Residues: 1-144; Coverage: 100%)
Gene names: EIF1A, EIF1AX, EIF4C
Sequence domains: Translation initiation factor 1A / IF-1
Small ribosomal subunit protein eS25 Chain: a
Small ribosomal subunit protein uS2 Chain: B
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS1 Chain: C
Small ribosomal subunit protein eS27 Chain: c
40S ribosomal protein S2 Chain: D
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS3 Chain: E
Small ribosomal subunit protein uS14 Chain: e
40S ribosomal protein S4 Chain: F
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: f
Small ribosomal subunit protein uS7 Chain: G
Ubiquitin-ribosomal protein eS31 fusion protein Chain: g
40S ribosomal protein S6 Chain: H
Small ribosomal subunit protein RACK1 Chain: h
Small ribosomal subunit protein eS7 Chain: I
Small ribosomal subunit protein eS8 Chain: J
Small ribosomal subunit protein eS32 Chain: n
Small ribosomal subunit protein uS4 Chain: K
Eukaryotic translation initiation factor 5B Chain: x
Small ribosomal subunit protein eS10 Chain: L
Small ribosomal subunit protein uS17 Chain: M
Small ribosomal subunit protein eS12 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein uS11 Chain: P
Small ribosomal subunit protein uS19 Chain: Q
Small ribosomal subunit protein uS9 Chain: R
Small ribosomal subunit protein eS17 Chain: S
Small ribosomal subunit protein uS13 Chain: T
Small ribosomal subunit protein eS19 Chain: U
Small ribosomal subunit protein uS10 Chain: V
Small ribosomal subunit protein eS21 Chain: W
Small ribosomal subunit protein uS8 Chain: X
Small ribosomal subunit protein uS12 Chain: Y
18S Ribosomal RNA Chain: 2
Met-tRNA-i-Met Chain: i
HCV IRES Chain: z

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-25542
Expression system: Escherichia coli