› 7sza

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of human PRPK complex with a compound

Released:
DOI: 10.2210/pdb7sza/pdb
PDB entry 7sza coloured by chain, front view.
PDB entry 7sza coloured by chain, side view.
PDB entry 7sza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Development of PRPK Directed Phthalimides
Seo H-S, Mizutani T, Hideshima T, Vangos NE, Zhang T, Anderson KC, Gray NS, Dhe-Paganon S
Biorxiv (2021)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188746 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
EKC/KEOPS complex subunit TP53RK Chains: A, C
Molecule details ›
Chains: A, C
Length: 255 amino acids
Theoretical weight: 28.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96S44 (Residues: 1-253; Coverage: 100%)
Gene names: C20orf64, PRPK, TP53RK
Sequence domains: Lipopolysaccharide kinase (Kdo/WaaP) family
EKC/KEOPS complex subunit TPRKB Chains: B, D
Molecule details ›
Chains: B, D
Length: 178 amino acids
Theoretical weight: 19.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3C4 (Residues: 1-175; Coverage: 100%)
Gene names: CGI-121, My019, TPRKB
Sequence domains: Kinase binding protein CGI-121

Ligands and Environments

2 bound ligands:
Ligand DUI 2 x DUI
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 297.51Å b: 47.49Å c: 62.27Å
α: 90° β: 96.53° γ: 90°
R-values:
R R work R free
0.175 0.173 0.216
Expression system: Escherichia coli BL21(DE3)

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