› 7szh

X-ray diffraction
1.79Å resolution

Structure of the Rieske Non-heme Iron Oxygenase SxtT with beta-saxitoxinol Bound

Released:
DOI: 10.2210/pdb7szh/pdb
PDB entry 7szh coloured by chain, front view.
PDB entry 7szh coloured by chain, side view.
PDB entry 7szh coloured by chain, top view.
Source organism: Microseira wollei
Primary publication:
Design principles for site-selective hydroxylation by a Rieske oxygenase.
Liu J, Tian J, Perry C, Lukowski AL, Doukov TI, Narayan ARH, Bridwell-Rabb J
OpenAccess logo Nat Commun 13 255 (2022)
PMID: 35017498

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 334 amino acids
Theoretical weight: 38.43 KDa
Source organism: Microseira wollei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3RVQ0 (Residues: 1-334; Coverage: 100%)
Gene name: sxtT
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand FES 3 x FES
Ligand GOL 22 x GOL
Ligand D82 3 x D82
Ligand FE 3 x FE
Ligand SO4 6 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C222
Unit cell:
a: 153.282Å b: 158.47Å c: 115.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Rieske Oxygenase Catalyzed C-H Bond Functionalization Reactions in Chlorophyll b Biosynthesis.
Liu et al. (2022)
6 more

1 mention without citation

Engineering Rieske oxygenase activity one piece at a time.
Brimberry et al. (2023)