› 7t0o

Electron Microscopy
8.7Å resolution

cryoEM reconstruction of the HIV gp140 in complex with the extracellular domains of CD4 and the adnectin domain of Combinectin. The gp140 and CD4 coordinates from entry 6EDU were rigid body fitted to the EM map along withe the crystal structure of CD4+adnectin

Released:
DOI: 10.2210/pdb7t0o/pdb
PDB entry 7t0o coloured by chain, front view.
PDB entry 7t0o coloured by chain, side view.
PDB entry 7t0o coloured by chain, top view.
Source organisms:
Primary publication:
Novel Bent Conformation of CD4 Induced by HIV-1 Inhibitor Indirectly Prevents Productive Viral Attachment.
Wensel D, Williams S, Dixon DP, Ward P, McCormick P, Concha N, Stewart E, Hong X, Mazzucco C, Pal S, Ding B, Fellinger C, Krystal M
J Mol Biol 434 167395 (2022)
PMID: 34896364
Related structures: EMD-25581

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-134563 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Envelope glycoprotein gp160 Chains: D, G, I
Molecule details ›
Chains: D, G, I
Length: 650 amino acids
Theoretical weight: 72.83 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-508, 509-661; Coverage: 74%)
Gene name: env
Sequence domains:
BG505 SOSIP.664 gp140 Chain: B
Molecule details ›
Chain: B
Length: 39 amino acids
Theoretical weight: 3.34 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens
BG505 SOSIP.664 gp140 Chains: F, J
Molecule details ›
Chains: F, J
Length: 38 amino acids
Theoretical weight: 3.25 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens
T-cell surface glycoprotein CD4 Chains: H, L, M
Molecule details ›
Chains: H, L, M
Length: 370 amino acids
Theoretical weight: 41.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-389; Coverage: 84%)
Gene name: CD4
Sequence domains:
Adnectin Chains: O, P, Q
Molecule details ›
Chains: O, P, Q
Length: 108 amino acids
Theoretical weight: 11.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
BG505 SOSIP.664 gp140 Chains: K, N, R
Molecule details ›
Chains: K, N, R
Length: 35 amino acids
Theoretical weight: 3 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.7Å
Relevant EMDB volumes: EMD-25581
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 mention without citation

The molecular immune modulator adenosine deaminase-1 enhances HIV specific humoral and cellular responses to a native-like HIV envelope trimer DNA vaccine.
Kutzler et al. (2024)