› 7t1u

X-ray diffraction
2.65Å resolution

Crystal structure of a superbinder Src SH2 domain (sSrcF) in complex with a high affinity phosphopeptide

Released:
DOI: 10.2210/pdb7t1u/pdb
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PDB entry 7t1u coloured by chain, front view.
PDB entry 7t1u coloured by chain, side view.
PDB entry 7t1u coloured by chain, top view.
Source organisms:
Primary publication:
Engineered SH2 Domains for Targeted Phosphoproteomics.
Martyn GD, Veggiani G, Kusebauch U, Morrone SR, Yates BP, Singer AU, Tong J, Manczyk N, Gish G, Sun Z, Kurinov I, Sicheri F, Moran MF, Moritz RL, Sidhu SS
ACS Chem Biol 17 1472-1484 (2022)
PMID: 35613471

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146386 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12931 (Residues: 147-251; Coverage: 19%)
Gene names: SRC, SRC1
Sequence domains: SH2 domain
Synthetic phosphopeptide Chains: D, E
Molecule details ›
Chains: D, E
Length: 7 amino acids
Theoretical weight: 987 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 34.87Å b: 35.89Å c: 53.98Å
α: 75.85° β: 74.77° γ: 61.42°
R-values:
R R work R free
0.224 0.218 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

2 review citations

Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.
Jiang et al. (2024)
1 more

1 mention without citation

Engineered SH2 Domains for Targeted Phosphoproteomics.
Martyn et al. (2022)

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