› 7t21

Electron Microscopy
5.4Å resolution

E. coli DnaB bound to ssDNA and ADP-AlF4

Released:
DOI: 10.2210/pdb7t21/pdb
PDB entry 7t21 coloured by chain, front view.
PDB entry 7t21 coloured by chain, side view.
PDB entry 7t21 coloured by chain, top view.
Source organisms:
Entry authors: Oakley AJ, Xu ZQ
Related structures: EMD-25608

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122056 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Replicative DNA helicase DnaB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.45 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 1-471; Coverage: 100%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Sequence domains:
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 20 nucleotides
Theoretical weight: 6.04 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 5 x ADP
Ligand ALF 5 x ALF
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.4Å
Relevant EMDB volumes: EMD-25608
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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