› 7t23

Electron Microscopy
4.2Å resolution

E. coli DnaB bound to two DnaG C-terminal domains, ssDNA, ADP and AlF4

Released:
DOI: 10.2210/pdb7t23/pdb
PDB entry 7t23 coloured by chain, front view.
PDB entry 7t23 coloured by chain, side view.
PDB entry 7t23 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Oakley AJ, Xu ZQ
Related structures: EMD-25610

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216580 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Replicative DNA helicase DnaB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.41 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 1-471; Coverage: 100%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Sequence domains:
DNA primase Chains: G, H
Molecule details ›
Chains: G, H
Length: 148 amino acids
Theoretical weight: 16.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABS5 (Residues: 434-581; Coverage: 26%)
Gene names: JW3038, b3066, dnaG, dnaP, parB
Sequence domains: DNA primase DnaG DnaB-binding
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 20 nucleotides
Theoretical weight: 6.04 KDa

Ligands and Environments

3 bound ligands:
Ligand ADP 5 x ADP
Ligand ALF 5 x ALF
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-25610
Expression system: Escherichia coli BL21(DE3)

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