› 7t2h

Electron Microscopy
3.2Å resolution

CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)

Released:
DOI: 10.2210/pdb7t2h/pdb
PDB entry 7t2h coloured by chain, front view.
PDB entry 7t2h coloured by chain, side view.
PDB entry 7t2h coloured by chain, top view.
Source organisms:
Primary publication:
Insights into distinct signaling profiles of the µOR activated by diverse agonists.
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022)
PMID: 36411392
Related structures: EMD-25613

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225642 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Mu-type opioid receptor Chain: D
Molecule details ›
Chain: D
Length: 356 amino acids
Theoretical weight: 40 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 9-51, 52-358; Coverage: 88%)
  • Best match: P42866-14 (Residues: 26-290)
Gene names: Mor, Oprm, Oprm1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand EID 1 x EID
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-25613
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Opioid signaling and design of analgesics.
Paul et al. (2023)
7 more

5 mentions without citation

IUPHAR themed review: Opioid efficacy, bias, and selectivity.
Ramos-Gonzalez et al. (2023)
4 more