› 7t3e

X-ray diffraction
1.04Å resolution

Structure of the sialic acid bound Tripartite ATP-independent Periplasmic (TRAP) periplasmic component SiaP from Photobacterium profundum

Released:
DOI: 10.2210/pdb7t3e/pdb
PDB entry 7t3e coloured by chain, front view.
PDB entry 7t3e coloured by chain, side view.
PDB entry 7t3e coloured by chain, top view.
Source organism: Photobacterium profundum
Primary publication:
Structure and mechanism of a tripartite ATP-independent periplasmic TRAP transporter.
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
OpenAccess logo Nat Commun 14 1120 (2023)
PMID: 36849793

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP-type C4-dicarboxylate transport system, periplasmic component Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 33.93 KDa
Source organism: Photobacterium profundum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6LPV9 (Residues: 24-322; Coverage: 100%)
Gene names: PBPRA2281, SMB20295
Sequence domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand SLB 2 x SLB
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 58.596Å b: 87.313Å c: 129.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.124 0.139
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
1 other article

1 mention without citation

Structure and mechanism of a tripartite ATP-independent periplasmic TRAP transporter.
Davies et al. (2023)