› 7t3j

Electron Microscopy
3.2Å resolution

Cryo-EM structure of Csy-AcrIF24

Released:
DOI: 10.2210/pdb7t3j/pdb
PDB entry 7t3j coloured by chain, front view.
PDB entry 7t3j coloured by chain, side view.
PDB entry 7t3j coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor.
Mukherjee IA, Gabel C, Noinaj N, Bondy-Denomy J, Chang L
Nat Chem Biol (2022)
PMID: 36163386
Related structures: EMD-25660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-168819 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated endonuclease Cas6/Csy4 Chain: C
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
AcrIF24 Chains: J, K
RNA (61-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-25660
Expression system: Escherichia

Quick links

Citations

1 review citation

Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks.
Forsberg KJ. (2023)
2 other articles