› 7t4s

Electron Microscopy
3.1Å resolution

CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with NRP2 and neutralizing fabs 8I21 and 13H11

Released:
DOI: 10.2210/pdb7t4s/pdb
PDB entry 7t4s coloured by chain, front view.
PDB entry 7t4s coloured by chain, side view.
PDB entry 7t4s coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for HCMV Pentamer receptor recognition and antibody neutralization.
Kschonsak M, Johnson MC, Schelling R, Green EM, Rougé L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
OpenAccess logo Sci Adv 8 eabm2536 (2022)
PMID: 35275719
Related structures: EMD-25687

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-207848 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 87.31 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6SW67 (Residues: 1-715; Coverage: 96%)
Gene names: UL75, gH
Sequence domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 278 amino acids
Theoretical weight: 30.85 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HCH8 (Residues: 1-278; Coverage: 100%)
Gene names: UL115, gL
Sequence domains: Cytomegalovirus glycoprotein L
Envelope protein UL128 Chain: C
Molecule details ›
Chain: C
Length: 171 amino acids
Theoretical weight: 19.75 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: V9LLX6 (Residues: 1-171; Coverage: 100%)
Gene name: UL128
Envelope glycoprotein UL130 Chain: D
Molecule details ›
Chain: D
Length: 254 amino acids
Theoretical weight: 28.87 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HCP3 (Residues: 1-214; Coverage: 100%)
Gene name: UL130
Sequence domains: HCMV glycoprotein pUL130
Protein UL131A Chain: E
Molecule details ›
Chain: E
Length: 129 amino acids
Theoretical weight: 15.01 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HET4 (Residues: 1-129; Coverage: 100%)
Gene name: UL131A
Neuropilin-2 Chain: F
Molecule details ›
Chain: F
Length: 875 amino acids
Theoretical weight: 98.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O60462 (Residues: 1-864; Coverage: 93%)
  • Best match: O60462-5 (Residues: 1-813)
Gene names: NRP2, VEGF165R2
Sequence domains:
Fab 8I21 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab 13H11 heavy chain Chain: J
Molecule details ›
Chain: J
Length: 250 amino acids
Theoretical weight: 26.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab 13H11 light chain Chain: K
Molecule details ›
Chain: K
Length: 237 amino acids
Theoretical weight: 25.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab 8I21 light chain Chain: I
Molecule details ›
Chain: I
Length: 238 amino acids
Theoretical weight: 26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand NAG 9 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-25687
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

2 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
1 more