› 7t4s

Electron Microscopy
3.1Å resolution

CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with NRP2 and neutralizing fabs 8I21 and 13H11

Released:
DOI: 10.2210/pdb7t4s/pdb
PDB entry 7t4s coloured by chain, front view.
PDB entry 7t4s coloured by chain, side view.
PDB entry 7t4s coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for HCMV Pentamer receptor recognition and antibody neutralization.
Kschonsak M, Johnson MC, Schelling R, Green EM, Rougé L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
OpenAccess logo Sci Adv 8 eabm2536 (2022)
PMID: 35275719
Related structures: EMD-25687

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-207848 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 87.31 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6SW67 (Residues: 1-715; Coverage: 96%)
Gene names: UL75, gH
Sequence domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 278 amino acids
Theoretical weight: 30.85 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HCH8 (Residues: 1-278; Coverage: 100%)
Gene names: UL115, gL
Sequence domains: Cytomegalovirus glycoprotein L
Envelope protein UL128 Chain: C
Envelope glycoprotein UL130 Chain: D
Protein UL131A Chain: E
Neuropilin-2 Chain: F
Fab 8I21 heavy chain Chain: H
Fab 13H11 heavy chain Chain: J
Fab 13H11 light chain Chain: K
Fab 8I21 light chain Chain: I

Ligands and Environments

2 bound ligands:
Ligand NAG 9 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-25687
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

2 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
1 more