› 7t50

X-ray diffraction
1.9Å resolution

Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in chromate-bound form

Released:
DOI: 10.2210/pdb7t50/pdb
Model geometry
Fit model/data
PDB entry 7t50 coloured by chain, front view.
PDB entry 7t50 coloured by chain, side view.
PDB entry 7t50 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PA1
Primary publication:
The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis.
Maunders EA, Ngu DHY, Ganio K, Hossain SI, Lim BYJ, Leeming MG, Luo Z, Tan A, Deplazes E, Kobe B, McDevitt CA
OpenAccess logo Front Microbiol 13 903146 (2022)
PMID: 35685933

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tungstate/molybdate/chromate-binding protein ModA Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 24.38 KDa
Source organism: Pseudomonas aeruginosa PA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I2N2 (Residues: 21-251; Coverage: 100%)
Gene names: PA1863, modA
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

3 bound ligands:
Ligand CQ4 2 x CQ4
Ligand NH4 3 x NH4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 45.627Å b: 41.223Å c: 111.366Å
α: 90° β: 92.758° γ: 90°
R-values:
R R work R free
0.237 0.235 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

The molybdate-binding protein ModA is required for Proteus mirabilis-induced UTI.
Huang et al. (2023)

1 mention without citation

The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis.
Maunders et al. (2022)