› 7t66

X-ray diffraction
2.19Å resolution

Co-crystal structure of Chaetomium glucosidase with compound UV-4

Released:
DOI: 10.2210/pdb7t66/pdb
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PDB entry 7t66 coloured by chain, front view.
PDB entry 7t66 coloured by chain, side view.
PDB entry 7t66 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Identification of Endoplasmic Reticulum α-Glucosidase I from a Thermophilic Fungus as a Platform for Structure-Guided Antiviral Drug Design.
Karade SS, Kolesnikov A, Treston AM, Mariuzza RA
Biochemistry 61 822-832 (2022)
PMID: 35476408

Function and Biology Details

Reaction catalysed: 
Glc(3)Man(9)GlcNAc(2)-[protein] + H(2)O = Glc(2)Man(9)GlcNAc(2)-[protein] + beta-D-glucopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-oligosaccharide glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 819 amino acids
Theoretical weight: 93.32 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Homo sapiens
UniProt:
  • Canonical: G0SFD1 (Residues: 30-809; Coverage: 97%)
Gene name: CTHT_0061620
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GOL 2 x GOL
Ligand 6A9 2 x 6A9
Ligand SO4 10 x SO4
Ligand BTB 1 x BTB
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 135.587Å b: 178.395Å c: 180.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.231
Expression system: Homo sapiens

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Citations

1 review citation

Expanding horizons of iminosugars as broad-spectrum anti-virals: mechanism, efficacy and novel developments.
Liu et al. (2024)
1 other article

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