7t94

Electron Microscopy
3.16Å resolution

Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225091 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Muscarinic acetylcholine receptor M2 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(o) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Antibody fragment Chain: E

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.16Å
Relevant EMDB volumes: EMD-25751
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda