› 7t94

Electron Microscopy
3.16Å resolution

Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM

Released:
DOI: 10.2210/pdb7t94/pdb
PDB entry 7t94 coloured by chain, front view.
PDB entry 7t94 coloured by chain, side view.
PDB entry 7t94 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and dynamic insights into supra-physiological activation and allosteric modulation of a muscarinic acetylcholine receptor.
Xu J, Wang Q, Hübner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
OpenAccess logo Nat Commun 14 376 (2023)
PMID: 36690613
Related structures: EMD-25751

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225091 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Muscarinic acetylcholine receptor M2 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08172 (Residues: 4-232, 368-466; Coverage: 70%)
Gene name: CHRM2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(o) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P09471 (Residues: 1-354; Coverage: 100%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment Chain: E
Molecule details ›
Chain: E
Length: 256 amino acids
Theoretical weight: 27.34 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:
Ligand ACH 1 x ACH
Ligand 2CU 1 x 2CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.16Å
Relevant EMDB volumes: EMD-25751
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda

Quick links

Citations

2 review citations

G protein-coupled receptors (GPCRs): advances in structures, mechanisms, and drug discovery.
Zhang et al. (2024)
1 more

1 mention without citation

Allosteric modulation of G protein-coupled receptor signaling.
Shen et al. (2023)