› 7t9i

Electron Microscopy
2.9Å resolution

Native human TSH bound to human Thyrotropin receptor in complex with miniGs399 (composite structure)

Released:
DOI: 10.2210/pdb7t9i/pdb
PDB entry 7t9i coloured by chain, front view.
PDB entry 7t9i coloured by chain, side view.
PDB entry 7t9i coloured by chain, top view.
Source organisms:
Primary publication:
Autoantibody mimicry of hormone action at the thyrotropin receptor.
Faust B, Billesbølle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022)
PMID: 35940205
Related structures: EMD-25758

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224552 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (8 distinct):
Glycoprotein hormones alpha chain Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 25-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Thyrotropin subunit beta Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01222 (Residues: 21-132; Coverage: 95%)
Gene name: TSHB
Sequence domains: Cystine-knot domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 142 amino acids
Theoretical weight: 15.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Thyrotropin receptor Chain: R
Molecule details ›
Chain: R
Length: 702 amino acids
Theoretical weight: 79.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A0A0MTJ0 (Residues: 22-764; Coverage: 93%)
Gene name: TSHR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: X
Molecule details ›
Chain: X
Length: 261 amino acids
Theoretical weight: 30.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 204-394; Coverage: 46%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: Y
Molecule details ›
Chain: Y
Length: 370 amino acids
Theoretical weight: 40.79 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Z
Molecule details ›
Chain: Z
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 2 x NAG
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-25758
Expression systems:
  • Escherichia coli
  • Homo sapiens
  • Trichoplusia ni

Quick links

Citations

3 review citations

G protein-coupled receptors in cochlea: Potential therapeutic targets for hearing loss.
Ma et al. (2022)
2 more

4 mentions without citation

Crystal structure of LGR ligand α2/β5 from Caenorhabditis elegans with implications for the evolution of glycoprotein hormones.
Gong et al. (2023)
3 more