› 7taw

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the Csy-AcrIF24-promoter DNA dimer

Released:
DOI: 10.2210/pdb7taw/pdb
PDB entry 7taw coloured by chain, front view.
PDB entry 7taw coloured by chain, side view.
PDB entry 7taw coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor.
Mukherjee IA, Gabel C, Noinaj N, Bondy-Denomy J, Chang L
Nat Chem Biol (2022)
PMID: 36163386
Related structures: EMD-25788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-122062 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein Csy1 Chains: A, a
Molecule details ›
Chains: A, a
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chains: B, b
Molecule details ›
Chains: B, b
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated endonuclease Cas6/Csy4 Chains: C, c
Molecule details ›
Chains: C, c
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I, d, e, f, g, h, i
Molecule details ›
Chains: D, E, F, G, H, I, d, e, f, g, h, i
Length: 360 amino acids
Theoretical weight: 39.78 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02MM1 (Residues: 2-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
AcrIF24 Chains: J, K
Molecule details ›
Chains: J, K
Length: 228 amino acids
Theoretical weight: 25 KDa
Source organism: Pseudomonas
Expression system: Escherichia
DNA (5'-D(P*AP*TP*AP*GP*CP*TP*CP*GP*AP*TP*TP*CP*GP*AP*GP*CP*TP*AP*A)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 19 nucleotides
Theoretical weight: 5.83 KDa
DNA (5'-D(P*TP*TP*AP*GP*CP*TP*CP*GP*AP*AP*TP*CP*GP*AP*GP*CP*TP*AP*T)-3') Chain: X
Molecule details ›
Chain: X
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
RNA (61-MER) Chains: M, m
Molecule details ›
Chains: M, m
Length: 61 nucleotides
Theoretical weight: 19.54 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-25788
Expression system: Escherichia

Quick links

Citations

1 review citation

Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks.
Forsberg KJ. (2023)
2 other articles