› 7tax

Electron Microscopy
2.8Å resolution

Cryo-EM structure of the Csy-AcrIF24-promoter DNA complex

Released:
DOI: 10.2210/pdb7tax/pdb
PDB entry 7tax coloured by chain, front view.
PDB entry 7tax coloured by chain, side view.
PDB entry 7tax coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor.
Mukherjee IA, Gabel C, Noinaj N, Bondy-Denomy J, Chang L
Nat Chem Biol (2022)
PMID: 36163386
Related structures: EMD-25789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-122063 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas
Expression system: Escherichia
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated endonuclease Cas6/Csy4 Chain: C
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
AcrIF24 Chains: J, K
DNA (5'-D(P*AP*TP*AP*GP*CP*TP*CP*GP*AP*TP*TP*CP*GP*AP*GP*CP*TP*AP*A)-3') Chain: Y
DNA (5'-D(P*TP*TP*AP*GP*CP*TP*CP*GP*AP*AP*TP*CP*GP*AP*GP*CP*TP*AP*T)-3') Chain: X
RNA (61-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-25789
Expression system: Escherichia

Quick links

Citations

1 review citation

Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks.
Forsberg KJ. (2023)
2 other articles

1 mention without citation

Proteomic Analysis of Arachis hypogaea Seeds from Different Maturity Classes.
Cherry et al. (2024)