› 7tbf

Electron Microscopy
3.1Å resolution

Locally refined region of SARS-CoV-2 spike in complex with antibodies B1-182.1 and A19-61.1

Released:
DOI: 10.2210/pdb7tbf/pdb
PDB entry 7tbf coloured by chain, front view.
PDB entry 7tbf coloured by chain, side view.
PDB entry 7tbf coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for potent antibody neutralization of SARS-CoV-2 variants including B.1.1.529.
Zhou T, Wang L, Misasi J, Pegu A, Zhang Y, Harris DR, Olia AS, Talana CA, Yang ES, Chen M, Choe M, Shi W, Teng IT, Creanga A, Jenkins C, Leung K, Liu T, Stancofski ED, Stephens T, Zhang B, Tsybovsky Y, Graham BS, Mascola JR, Sullivan NJ, Kwong PD
OpenAccess logo Science 376 eabn8897 (2022)
PMID: 35324257
Related structures: EMD-25797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276083 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Spike protein S1 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 21.99 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 332-527; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
Heavy chain of SARS-CoV-2 antibody B1-182.1 Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Light chain of SARS-CoV-2 antibody B1-182.1 Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain of SARS-CoV-2 antibody A19-61.1 Chain: D
Molecule details ›
Chain: D
Length: 127 amino acids
Theoretical weight: 13.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Light chain of SARS-CoV-2 antibody A19-61.1 Chain: E
Molecule details ›
Chain: E
Length: 107 amino acids
Theoretical weight: 11.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-25797
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Broadly neutralizing antibodies to SARS-CoV-2 and other human coronaviruses.
Chen et al. (2023)
18 more

4 mentions without citation

Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron.
Teng et al. (2022)
3 more