› 7tcm

X-ray diffraction
1.95Å resolution

Crystal Structure of aspartate-semialdehyde dehydrogenase from Acinetobacter baumannii in complex with NADP

Released:
DOI: 10.2210/pdb7tcm/pdb
PDB entry 7tcm coloured by chain, front view.
PDB entry 7tcm coloured by chain, side view.
PDB entry 7tcm coloured by chain, top view.
Source organism: Acinetobacter baumannii
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 41.64 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: V5VGT0 (Residues: 1-372; Coverage: 100%)
Gene names: A7M90_00850, ABUW_3451, APD33_04490, AUO97_09570, B9W25_00650, B9X95_14805, C5U34_03210, CBE85_04990, CPI82_07390, CV954_017635, DOL94_07895, DWA16_15990, EA706_08250, EA722_00480, EJ062_04155, F2P40_11035, F4T83_05655, FJU42_00545, FPK81_09475, FQZ18_16955, G3N53_02155, GNY86_01050, IAG11_00475, IHV20_05060, IMO23_02025, J6E47_17230, JHZ39_000151, LV35_03159, MKP18_000688, P9867_002140, P9867_00495, SAMEA104305318_00176, SAMEA104305343_00905, SAMEA4394745_00151, asd
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 82.95Å b: 53.21Å c: 87.33Å
α: 90° β: 102.358° γ: 90°
R-values:
R R work R free
0.153 0.152 0.184
Expression system: Escherichia coli BL21(DE3)

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