› 7tdm

Electron Microscopy
6.9Å resolution

CryoEM Structure of sFab COP-2 Complex with human claudin-4 and Clostridium perfringens enterotoxin C-terminal domain

Released:
DOI: 10.2210/pdb7tdm/pdb
PDB entry 7tdm coloured by chain, front view.
PDB entry 7tdm coloured by chain, side view.
PDB entry 7tdm coloured by chain, top view.
Source organisms:
Primary publication:
Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
Orlando BJ, Dominik PK, Roy S, Ogbu CP, Erramilli SK, Kossiakoff AA, Vecchio AJ
OpenAccess logo J Biol Chem 102357 (2022)
PMID: 35952760
Related structures: EMD-25834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-224873 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Claudin-4 Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 22.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O14493 (Residues: 1-209; Coverage: 100%)
Gene names: CLDN4, CPER, CPETR1, WBSCR8
Sequence domains: PMP-22/EMP/MP20/Claudin family
Heat-labile enterotoxin B chain Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.11 KDa
Source organism: Clostridium perfringens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01558 (Residues: 192-319; Coverage: 40%)
Gene name: cpe
COP-2 Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
COP-2 Fab Light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.9Å
Relevant EMDB volumes: EMD-25834
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Computational design of a chimeric toxin against Claudin-4-expressing cancer cells: molecular modeling, docking and molecular dynamics simulation analysis.
Safaei et al. (2023)

2 mentions without citation

Multiscale modelling of claudin-based assemblies: A magnifying glass for novel structures of biological interfaces.
Berselli et al. (2022)
1 more