› 7tdy

X-ray diffraction
1.53Å resolution

The ubiquitin-associated domain of human thirty-eight negative kinase 1, flexibly fused to the 1TEL crystallization chaperone via a 2-glycine linker and crystallized at low protein concentration

Released:
DOI: 10.2210/pdb7tdy/pdb
PDB entry 7tdy coloured by chain, front view.
PDB entry 7tdy coloured by chain, side view.
PDB entry 7tdy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain.
Nawarathnage S, Tseng YJ, Soleimani S, Smith T, Pedroza Romo MJ, Abiodun WO, Egbert CM, Madhusanka D, Bunn D, Woods B, Tsubaki E, Stewart C, Brown S, Doukov T, Andersen JL, Moody JD
Structure (2023)
PMID: 37776857

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-receptor tyrosine-protein kinase TNK1; Transcription factor ETV6 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41212 (Residues: 47-120; Coverage: 16%)
  • Canonical: Q13470 (Residues: 590-666; Coverage: 12%)
Gene names: ETV6, TEL, TEL1, TNK1
Sequence domains: Sterile alpha motif (SAM)/Pointed domain

Ligands and Environments

1 bound ligand:
Ligand FMT 6 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P65
Unit cell:
a: 67.221Å b: 67.221Å c: 60.888Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.217
Expression system: Escherichia coli BL21(DE3)

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